Condyloma acuminatum (CA) is one of the most common sexually transmitted diseases caused by human papillomavirus (HPV) infection. This virus causes not only inflammation-induced benign squamous hyperplasia, as in anal CA, but also anal intraepithelial neoplasia, which is a precursor of anal squamous cell carcinoma (SCC). Therefore, observation of anal CA with various modalities can help us decide on a treatment strategy and prevent anal SCC. Here, we report a case of anal CA, for which endocytoscopy was used to provide the ultra-high-magnification images at cellular resolution in real time with manipulating zoom lever after staining.